Vimentin anticorps (FITC)

N° du produit ABIN1042493

L’anticorps Souris Monoclonal anti-Vimentin a été validé pour WB, FACS, IHC (p), ICC et IHC (fro). Il convient pour détecter Vimentin dans des échantillons de Humain, Souris, Rat, Porc, Poulet, Singe, Chien, Chévre et Poisson zèbre (Danio rerio). Il y a 5+ publications disponibles.

Aperçu rapide pour Vimentin anticorps (FITC) (ABIN1042493)

Antigène: Vimentin (VIM)

Reactivité: Humain, Souris, Rat, Porc, Poulet, Singe, Chien, Chévre, Poisson zèbre (Danio rerio)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Vimentin est conjugé à/à la FITC

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: RV202

Détail du produit anti-Vimentin anticorps (FITC)

Specificité: RV202-FITC reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma. RV202-FITC is suitable for immunocytochemistry on cell cultures and cytospins, immuno-histochemistry on frozen tissues, as well as for flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:10.

Attributs du produit: Cytoskeletal,Tumour marker

Purification: Purified

Isotype: IgG1

Information d'application

Indications d'application: RV202 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissues and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:100 – 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:1000 for immunoblotting applications.

Commentaires:

RV202 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS containing 0,1% BSA, 0.09% sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Références pour anticorps anti-Vimentin (FITC) (ABIN1042493)

Raats, Pieper, Vree Egberts, Verrijp, Ramaekers, Bloemendal: "Assembly of amino-terminally deleted desmin in vimentin-free cells." dans: The Journal of cell biology, Vol. 111, Issue 5 Pt 1, pp. 1971-85, (1990) (PubMed).

Ramaekers, van Niekerk, Poels, Schaafsma, Huijsmans, Robben, Schaart, Vooijs: "Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas." dans: The American journal of pathology, Vol. 136, Issue 3, pp. 641-55, (1990) (PubMed).

Pieper, Schaart, Krimpenfort, Henderik, Moshage, van de Kemp, Ramaekers, Berns, Bloemendal: "Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells." dans: The Journal of cell biology, Vol. 108, Issue 3, pp. 1009-24, (1989) (PubMed).

Viebahn, Lane, Ramaekers: "Keratin and vimentin expression in early organogenesis of the rabbit embryo." dans: Cell and tissue research, Vol. 253, Issue 3, pp. 553-62, (1988) (PubMed).

Ramaekers, Huysmans, Schaart, Moesker, Vooijs: "Tissue distribution of keratin 7 as monitored by a monoclonal antibody." dans: Experimental cell research, Vol. 170, Issue 1, pp. 235-49, (1987) (PubMed).

Détails sur Vimentin

Antigène: Vimentin (VIM)

Autre désignation: Vimentin

Sujet: Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.

Pathways: Caspase Cascade in Apoptosis